Aseptic operation in fermentation process

In the fermentation process, aseptic operation is very important, which can prevent the pollution of external miscellaneous bacteria and ensure that the fermentation process is not disturbed by any external factors, so as to obtain high-quality fermented products. The following are aseptic operations during fermentation:

1. Disinfection of equipment and tools. Before fermentation, it is necessary to ensure that the equipment and tools are thoroughly disinfected and stored in a sterile way.

2. Air filtration. The air filter is used to filter the miscellaneous bacteria in the air and prevent the miscellaneous bacteria from entering the fermentation operation area.

3. Wear sterile clothes. During the fermentation operation, you must wear sterile clothes and gloves and disinfect them with ultraviolet rays.

4. Prepare the culture medium. When preparing the culture medium, it is necessary to use a high-temperature autoclave for sterilization to ensure the sterility of the culture medium.

5. Aseptic sampling. In the fermentation process, aseptic sampling is carried out to avoid bacteria and other impurities polluting the sample.

6. Aseptic transfer. Aseptic transfer is required during operation, using sterile medical syringe or sterile culture bottle cap for transfer.