What does fsc ssc mean?

FSC and SSC are terms often heard in quality inspection. FSC refers to the forward scattered signal, while SSC refers to the lateral scattered signal. They are one of the commonly used parameters in flow cytometry detection. FSC refers to the forward scattering of light beams by cells, which distinguishes small particles from large particles. SSC is a parameter to detect the structure and complexity of cells, and different cell groups can be distinguished by light scattering of cell protoplasm.

Flow cytometry is an indispensable technology in modern life science, which detects the number and characteristics of different cells in samples, including FSC and SSC, through special fluorescent probes. This technology can be used for quantitative and qualitative analysis of cells and particles in tumor, immunity, blood, aquaculture, environmental science and food industry. Among them, FSC and SSC are both powerful parameters in flow cytometry, which can deeply analyze the anatomical characteristics of different particles and effectively distinguish different organisms in samples.

Although FSC and SSC play an important role in flow cytometry detection, in practice, different sample types have different requirements for parameter adjustment. For example, for large particles, properly reducing the event counting rate and enhancing SSC sensitivity will help improve the detection accuracy. For small particles in liquid, the FSC threshold can be lowered appropriately to improve the detection rate. Therefore, the rationality of FSC and SSC parameter adjustment has an important influence on the accuracy of the detection results, and it is also an essential link in the detection process of flow cytometry.