How is the polypeptide purified?

Polypeptide purification generally uses reversed-phase columns (such as C8, C 18, etc. )，2 14nm。 Buffer system is usually TFA-containing solvent with pH2.0, buffer A is DDH2O with 0. 1% TFA, and buffer B is 1%TFA/ACN/pH2.0, which is dissolved with buffer A before purification; If the solution is not good, dissolve it with buffer B and then dilute it with buffer A; For peptides with strong hydrophobicity, it is sometimes necessary to add a small amount of formic acid or acetic acid. The crude polypeptide was analyzed by HPLC. If the polypeptide is not long (below 15aa), there will generally be a main peak, which is generally a full-length product. For long peptides above 20aa, if there is no main peak, HPLC should match the mass to determine the molecular weight, and then determine which peak is the polypeptide to be synthesized.