Translation help of pharmaceutical English literature

Detoxification vitamin tablet (approval number: National Medicine Zhunzi Z20026566) is composed of Wei compound ingredients, and its main function is "clearing away heat and toxic materials, tonifying liver and kidney". In order to better guide the clinical treatment, according to the symptoms and medication characteristics of leukemia drugs, 27 pharmacological literatures related to leukemia and all oral compounds related to experimental drugs, including oral administration, gastric perfusion and in vitro experiments in mice, were searched, and the therapeutic effects of the drugs used in the compound and the experimental drugs on leukemia were discussed. The results show that six flavors can kill leukemia cells, 10 can enhance immune function, three flavors can improve hematopoietic system, and eight flavors can inhibit the growth of pathogenic microorganisms and resist fatigue. This provides further evidence for the clinical application of "Jiedu Weikang Tablet".

Key words: "Jiedu vitamin tablets", pharmacology, leukemia, immune function, blood system, pathogenic microorganisms, anti-fatigue, review.

Leukemia is a malignant hematopoietic system disease. It is characterized by unlimited abnormal development of bone marrow or other blood tissues, white blood cells and a series of immature cells (called leukemia cells). Invasion of human organs and blood into liquid causes changes in clinical manifestations and peripheral blood leukocytes. In order to get patients out of their misery as soon as possible, according to the pharmacodynamic research requirements of "eliminating pathogenic factors, strengthening body resistance, enhancing efficiency and detoxicating", we have made literature research on 627 components in the three herbs of Weikang Jiedu prescription, which are summarized as follows:

Antitumor effect

(1) "Guanzhong" in Northeast China: This extract has inhibitory effect on tumors of various transplanted animals. Studies show that Guanzhong B is an effective anti-tumor site. Membrane oxygen electrode detection showed that Guanzhong B P 388 significantly reduced the oxygen consumption rate of leukemia cells, and mitochondria may be a target cell for its anti-tumor effect [1]. The extract of northeast Guanzhong inhibits DNA synthesis in northeast Guanzhong, and is effective in Lewis lung cancer and P 388 leukemia in mice This compound is used as a substitute of "Mian Ma Guanzhong" belonging to the same family, and has been included in the Pharmacopoeia of 2000.

(2) Indigo Naturalis: Indirubin, an active ingredient, has anti-tumor activity and is an effective drug for treating chronic myeloid leukemia (CML) in clinic. Using radionuclide labeling (3 H-TdR, 3 H-uR, 3h- Leucine) and adding tumor DNA, RNA and protein respectively, the study shows that indirubin can inhibit the DNA metabolism and synthesis of leukemia cells in patients with chronic myeloid leukemia and acute myeloid leukemia, solid tumor of W 256 in rats, liver of mice and EAC cancer cells in ascites, slightly inhibit RNA synthesis, and have no obvious effect on protein synthesis [3-5].

(3) Hedyotis diffusa: its crude preparation has inhibitory effect on EAC cancer, Yoshida sarcoma, leukemia and other cancer cells only at high concentration in vitro [6-8].

(4) Scutellaria barbata: The blue screening test tube experiment showed that the tablet had a slight hemocyte inhibition effect on acute leukemia cells (AML), and the screening experiment with respiratory protective mask showed that the hemocyte inhibition rate on AML was over 75% [9].

(5) Wu Mei: The main component of this compound has certain curative effect on chronic tumors such as cervical cancer. In order to further confirm the anti-tumor activity and effective mechanism of its components, in vitro experiments were conducted with human primitive megalobocyte (HIM eg) and human promyelocytic leukemia cell (HL 60) as the research objects. From the counting of living cells and cloning experiments, it can be seen that the water extract and alcohol extract have a direct inhibitory effect on the growth of HIMeg and HL 60 cells [10].

(6) Artesunate: The cytotoxicity of artesunate B-compounds A, B, C and D of artesunate and its derivatives on various cell lines was studied by exclusion method and in vitro cloning method. When the concentration of these four compounds is 5 μ g/ml, their inhibitory rate on leukemia cells of P 388 mice is over 85% [1 1]. The hydroxyl group in the derivatives of artesunate (Ⅰ) was purchased into (Ⅱ, Ⅲ) by the difference of a pair of hexacyclic alkene-α-lactones, and Ⅱ was reduced to a heterogeneous ester (Ⅳ, Ⅴ), and Ⅱ was further oxidized to a post-hydroxyl-lactone (Ⅴ). Compounds Ⅱ, Ⅲ and ⅵ have anti-tumor activity on small P 388 leukemia cells in vitro, while the inhibitory rates of Ⅰ, ⅵ and ⅵ on P 388 cells are 10 μ g/ml and 1 μ g/ml, respectively. The inhibition rates of ⅵ were 100% and 47% respectively.

immunologic function

(1) Indigo Naturalis: Indirubin 200 mg/kg sc, its effective component, for 6 days, enhanced the phagocytosis of chicken red blood cells by macrophages in tumor-bearing rats [12].

(2) Hedyotis diffusa: mice were given 0,6g (pharmacognosy) of its crude water extract/mouse, so as to enhance the ability of peritoneal fluid to phagocytize Staphylococcus albus leukemia [13]. In the aspect of phagocytosis of bacterial interleukin, the ability of rabbits fed with water decoction increased by more than three times compared with the control group [14, 15]. In vitro tests can enhance the phagocytosis of white blood cells and Staphylococcus aureus in human blood [16].

(3) Scutellaria barbata: The polysaccharide obtained from this product can promote the transformation of mouse spleen lymphocytes induced by concanavalin A (ConA) in vitro, and the optimal concentration is 400 r/ml [17].

(4) Astragalus membranaceus: 50% water decoction, 0.5ml/ig, can accelerate mice's participation in the dissection of plasma protein marker 13 1I, which shows that IR tissue has great love for liver. R and spleen cells can enhance phagocytosis [18]; 0.6 ml/onlig of this product 100% decoction can promote the addictive effect of mice on the red blood cells of the drug giant chicken [19]; The decoction of this product is 25 g/kg ig, which can enhance the phagocytosis of macrophages [20]; The decoction of this product 10 μ g/ml was injected into the skin of rats to culture tumor patients, which obviously enhanced the local graft-versus-host reaction, indicating that this product can promote the immune function of T cells [2 1].

(5) Lycium barbarum L.: An over-dose sheep red blood cell (SRBC) immunoassay (SOI) showed that Lycium barbarum L. polysaccharide had obvious inhibitory and regulatory effects on T cells (Ts) in aged mice and enhanced the activity of Ts cells [22]. It was confirmed by Yc rosette formation rate and EA rosette formation rate that LBP 10mg/kg was given to mice by gavage for 7 days, which could significantly increase the number and vitality of C 3b and Fc receptors in macrophages, and could weaken the inhibitory effect of hydrocortisone on C 3b and Fc receptors in macrophages [23].

(6) Artemisinin: its suspension of 450 mg/kg ip for 6 days continuously has the effect of sensitizing sheep red blood cells in mice and enhancing delayed hypersensitivity; 300 ~ 600mg/kg ip, the specific immunity of rose inhibited the flower formation of mice and the formation of hemolytic plaque, and the suspension of 0.56, 2.8 and 5.6mg/ml promoted lymphocyte transformation in vitro, promoting humoral immunity and suppressed immune cells [24].

(7) Fuling Tucheng; Humoral immune response is not inhibited, but cellular immune response can be selectively inhibited. Its water extract has a stronger effect on the attack after antigen sensitization and the reaction of contact dermatitis and sheep red blood cells (SRBC) in mice after treatment. In addition, this product has no obvious effect on the formation of SRBC cell antibodies in mice, but its hemolytic plaque is obviously more than that in the control group. At the same time, the serum hemolysin level has not decreased, and it is on the rise [25]. The water and alcohol extracts of Poria cocos compound can inhibit the swelling of egg white and carrageenan foot in rats [26].

(8) Cistanche deserticola: oral prednisolone decoction can enhance the humoral and cellular immune function of mice with yang deficiency [27]; Enhance the phagocytosis of mononuclear macrophages [28]; Its water extract (50 mg/kg, 100 mg/kg) can obviously increase the weight of spleen and thymus, enhance the phagocytic function of macrophages, increase the hemolysin and hemolytic plaque value, improve the lymphocyte transformation rate, increase the incorporation of 3 H-TdR into lymphocytes, enhance the delayed hypersensitivity of mice, increase the level of CAMP in peritoneal macrophages, reduce the level of CGMP, and make camp.

(9) Semen Cuscutae: its extract of 65438±0.6/kg ig for 6 days can enhance the phagocytic function of macrophages in burned mice, increase serum antibody hemolysin and promote the proliferation of spleen cells ConA [30].

(10), octupole day: taking orally 64g/kg/day of Jinji can make the thymus gland of minors atrophy, and taking orally10g/kg/day can also make the thymus gland atrophy of minors [3/kloc- Its decoction, administered for 65438 00 days, can inhibit thymus atrophy and increase the number of white blood cells in the blood [32].

. The role of microbial resistance

(1), Mian Ma Guanzhong: The decoction was diluted in test tubes, and 1: 800 to 1: 160 had different degrees of inhibition on various influenza viruses [33]; Its decoction has a strong effect on influenza A virus PR8 strain and Asian virus with the concentration of 1: 104- 105 in chicken experiments [34].

(2) Indigo Naturalis: 0.5 g/ml ethanol extract has white inhibitory effect on Bacillus anthracis, Pneumonia, Shigella dysenteriae, Vibrio cholerae, Staphylococcus aureus and Staphylococcus aureus in vitro [35]; Microsporum pilosum, Xu Anjun, red Xu Anjun and floc, one of the effective components, inhibited epidermal Xu Anjun, and the minimum inhibitory concentration was 5 μ g/ml [36].

(3) Hedyotis diffusa: The antibacterial activity in vitro is not significant, but only has a weak effect on Staphylococcus aureus and Shigella [37]. High concentration of decoction can inhibit the growth of Pseudomonas aeruginosa, typhoid Bacillus and Proteus, and many other common pathogenic effects are weak [38]. The effect of appendicitis in rabbits is better [39].

(4) Scutellaria barbata: 50% water decoction can inhibit Pinggou, Staphylococcus aureus, Shigella, typhoid, Pseudomonas aeruginosa and Escherichia coli [40].

(5) Wu Mei: In vitro screening showed that it inhibited many pathogens, such as Escherichia coli-Shigella dysenteriae, typhoid Bacillus, paratyphoid Bacillus, pertussis Bacillus, meningococcus, etc. [4 1-45].

(6) Astragalus membranaceus: decoction 50% 0.4 ml/onlig, for 2 days, and continued to take it for 5 days after being attacked by parainfluenza virus type I (Sendai) bb 1 strain, which has a protective effect on mice and can obviously reduce the mortality rate of mice [46]. A decoction with a concentration of 0.3%. Sindbis virus, Newcastle disease virus, follicular stomatitis virus and influenza virus have antiviral activity [47].

(7) Artemisinin: water suspension 1.8, 3.6, 5.4mg/embryo inhibited the embryo infected with influenza virus A 3-Beijing segment 79-2 [48]. The LC 50 (mg/ml) of Staphylococcus aureus, Escherichia coli, Streptococcus beta and Pneumococcus detected by agar plate method were 17.6, 13 1.9 and 263.9 respectively. 200: The ethanol extracts were 41.7,263.9, 14 1.4 and 93.3. The commercial ethanol extracts were 32. 1, 93.3, 70.7,107./respectively.

The minimum inhibitory concentrations of this drug acid to Staphylococcus aureus, Staphylococcus albus, Enterococcus and Bacillus subtilis were 65438 0.0, 65438 0.0, & gt2.0 and 0.5 mg/ml, respectively [49]. The flooding concentration of soft artesunate 1: 3 has different degrees of inhibition on Huang's, O 'Donnell's Bacillus and Kanu's dermatophytes in vitro [50].

(8) Morinda officinalis field: ethanol extract, inhibiting Bacillus subtilis [5 1] in vitro. The ethanol extract of Morinda officinalis how to inhibit HBV in vitro [52].

. Influence of hematopoietic system

(1) Astragalus membranaceus: The decoction of 20 g/kg, 65438±00d days in total, has the function of enriching blood for anemia and hemorrhagic hemolytic anemia caused by acetylcholine phenylhydrazine, can increase red blood cells and hemoglobin, promote the recovery of leukopenia and thrombocytopenia caused by cyclophosphamide, and can increase the number of reticulocytes and bone marrow cells [53].

(2) Lycium barbarum L.: Normal mice take 0.5 ml of ig 10% decoction continuously every month for 10 days, and the WBC will increase. The WBC of healthy people and cancer patients can be significantly increased by taking 50 g/d of po Lycium barbarum nuts and taking 1d continuously [54, 55]. LBP10 mg/(kg d) IP for 3 days can promote the proliferation of mouse bone marrow hematopoietic stem cells, significantly increase the number of tablet progenitor cells and promote their differentiation into tablets [56].

(3) Cuscuta chinensis: inhibiting the growth promotion of CFU-D [57].

. Anti-fatigue effect

(1) Wu Mei: Dry goods can eliminate fatigue. Citric acid is an indispensable substance in the process of energy conversion in the body. During physical fatigue, lactic acid in the blood is decomposed into CO 2 and H 2 O and excreted, thus preventing lactic acid from combining with muscle protein and avoiding cell and vascular sclerosis [58].

(2) The pharmacological experiments of Cistanche deserticola decoction were carried out in vitro, which showed that it inhibited the formation of LPO in rat liver homogenate and had strong anti-fatigue and anti-hypoxia effects [59]. Cistanche deserticola decoction can prolong the swimming time of mice and increase the decrease rate of serum creatine kinase under exercise load, which shows that its increase in physical fitness is based on its anti-fatigue effect [60].

Conclusion:

Through the analysis of Fang Yaozhong Sanweishi, an "antidote vitamin tablet", the following five effects are summarized:

1. Drugs that affect immune function are: Indigo Naturalis, Hedyotis diffusa, Scutellaria barbata, Astragalus, Lycium barbarum, artesunate, Smilax glabra, Cistanche deserticola, Cuscuta chinensis and Morinda officinalis.

2. Eight traditional Chinese medicines have inhibitory effects on pathogenic microorganisms, namely, Ma Guanzhong, Indigo Naturalis, Hedyotis diffusa, Scutellaria barbata, Wu Mei, Astragalus, Artesunate and Morinda officinalis.

3. Six drugs for killing leukemia cells, namely: Ma Guanzhong, Indigo Naturalis, Hedyotis diffusa, Scutellaria barbata, Wu Mei and artesunate.

4. Three flavors of Chinese medicine on hematopoietic function, namely: Astragalus membranaceus, Lycium barbarum and Cuscuta chinensis.

5. Anti-taste fatigue drugs are: Mume, Cistanche deserticola.

According to this analysis, it provides reference for further clinical application of Jiedu vitamin tablets.

I'll give you an English pharmacological literature network, which contains what you need! :/guowaiweb/toppage2 1.htm