How to identify how dna is made?

1, DNA extraction

The DNA contained in the sample nucleus is extracted and then purified to remove impurities in the sample.

2.PCR amplification

The Chinese name of PCR is polymerase chain reaction. Simply put, the step of PCR amplification is to copy a large number of fragments we need on the PCR instrument through enzymatic reaction and enlarge them to the extent that some special instruments can see them.

3. Post 3.PCR reaction

This step is mainly the preparation stage of ABI sequencer detection. Open the double-stranded DNA and add some internal standards for detection, which are mainly used to mark the length of the detection fragment.

4, capillary sequencer detection

Because DNA is charged, capillary electrophoresis has different electrophoresis speeds for different fragments of DNA. Under the same voltage and electrophoresis time, the swimming distance is different. These different distances can be distinguished by the internal standard measurement added in the early stage, and displayed on the computer through certain software, which is convenient for inspectors to process and analyze the data.

5. Analyze the data and publish the report.

It is mainly for inspectors to analyze, summarize and calculate the obtained results, and then make appraisal conclusions and reports.

Extended data:

Procedures required for paternity test of immigrants:

1. When applying for paternity test of immigrants, the authenticated person needs to provide documents that can prove his identity (such as ID card, household registration book, birth certificate, etc.). , only one can be provided);

2. Some countries need to be entrusted by notary offices to apply for visas. If required, please ask the notary office to provide a power of attorney;

Immigrant paternity test process:

1, the appraiser signs the power of attorney for judicial appraisal and collects samples in the appraisal center. If he can't go to the appraisal center in person, he can also collect samples and mail them by himself.

2. The collected samples will be sent to the laboratory for DNA testing, and the appraiser can wait for the appraisal report.

3. The appraisal center will generally issue an appraisal report within 5 working days.

It is suggested that the appraiser communicate with the customer service staff of the appraisal center in advance when doing the paternity test of immigrants, and the customer service staff will help the appraiser with professional knowledge at the first time.

References:

Baidu encyclopedia-paternity test