What plants can be used for tissue culture?

What plants can be used for tissue culture? Almost all plants can be propagated by tissue culture, but the required conditions and techniques are very high.

Now plant tissue culture technology has been widely used in production, such as orchids, cymbidium cymbidium, calla lilies and flamingos. Pitaya, banana, apple, jujube and pomegranate in fruits; Bamboo, eucalyptus and teak in forest trees; Siraitia grosvenorii, Gynostemma pentaphyllum and Dendrobium in medicinal materials; There are many broccoli, taro, potatoes and so on in vegetables.

What are the plants suitable for tissue culture? In theory, any plant can be cultured in tissue culture, but the purpose of tissue culture technology is to obtain a large number of excellent plants quickly, so it is necessary to choose plants with better morphology for culture. Plants' tissues and organs, stems, leaves and buds can all be cultured, and different tissues and organs should be selected according to different plants. You can also cultivate aseptic seedlings with seeds and then inoculate them.

Economy depends on what kind of plants you want to buy. If the plants you want to cultivate are expensive, you can not consider this. If you want to do a simple culture experiment, you can take local materials and randomly select a few plants to take their tender parts for culture.

What are the plants commonly used in tissue culture? First of all, it is obvious that the application of tissue culture is

1 rapid propagation of plants, the purpose of this application is to maintain the original excellent characteristics of plants, so this method is suitable for those plants with excellent characteristics, but it is difficult to reproduce asexually in the natural state, such as orchids.

2, plant detoxification: Because the virus will reduce the yield, it is used to detoxify crops, such as potatoes. Crops such as strawberries.

3. Production of cell products: It is mainly used for endangered plants, and the substances contained in plants play a great role. Therefore, after plant tissue culture callus, a large number of products are produced, and the production cycle is relatively short. Such as the production of paclitaxel.

The most commonly used plants in production are potatoes, strawberries, bananas, sugar cane, sweet potatoes, medlar, grapes, poplars, orchids, roses, lilies and carnations. There are also various newly introduced famous, special and excellent varieties and some precious medicinal materials.

The first question about plant tissue culture is dedifferentiation. Plant tissue culture usually adopts fully differentiated mature tissues, such as roots, stems and leaves. These fully differentiated explants must be dedifferentiated or dedifferentiated into undifferentiated callus. At this time, you can make a choice:

1, when the content of growth hormone is greater than cytokinin, it will promote the growth of "root primordium", that is, Mr. Root.

2. When the content of cytokinin is greater than growth hormone, it will promote the growth of "bud primordium", that is, it will sprout first.

That is, you can ask Mr. Corpus Callosum Root or Mr. Bud.

About plant tissue culture ... meristem cells, such as buds, are usually used as explants because these cells have low differentiation and rarely carry viruses.

The morphological structure of cells will change, which can be summarized as dedifferentiation and redifferentiation.

The nutrients are not always the same. First, plants are constantly being consumed, and sometimes we will supplement them. Second, it is necessary to adjust the concentration of hormones when inducing dedifferentiation and redifferentiation.

In plant tissue culture, tissue culture with pollen is considered as asexual reproduction (fertilized eggs are not fused from sperm eggs), and haploids are obtained by culture (directly developed from gametes)

One of the differences between plant tissue culture and plant cell culture is that plant tissue culture uses many cells, while plant cell culture uses one cell.

Secondly, maternal plants can be obtained by plant tissue culture, while maternal plants and haploid plants (such as pollen culture) can be obtained by plant cell culture.

What are the factors that affect tissue culture? 1. Plant materials.

(1) The difficulty of culture varies greatly with different plant tissues. For example, tissue culture of tobacco and carrot is easy, but bud induction of Lycium barbarum callus is difficult.

(2) The same plant material will also affect the experimental results because of the organ source and its physiological state, the age of the material and the length of storage time.

2. Culture medium

In vitro plant tissues and cells have relatively special requirements for nutrition and environmental conditions, so it is necessary to prepare suitable culture media. MS medium is a common medium in plant tissue culture, and its main components include:

(1) inorganic nutrition:

Macroelements: besides carbon, hydrogen and oxygen, there are also nitrogen, sulfur, phosphorus, magnesium, potassium and calcium.

Trace elements: iron, manganese, boron, zinc, copper, molybdenum, cobalt, iodine, etc.

(2) Organic nutrition

Vitamins, Aa, agar, activated carbon, nicotinic acid, inositol, sucrose, etc.

(3) Plant hormones

Auxin and cytokinin are key hormones to start cell division, dedifferentiation and redifferentiation. In the presence of auxin, the role of cytokinin shows a trend of strengthening. Using these two hormones in different order will get different experimental results.

When these two hormones are used at the same time, the proportion of their dosage affects the development direction of plant cells.

(4) Energy and osmotic pressure

Usually, plants themselves produce sugar by photosynthesis, and there is no need for external supply of sugar. However, under the condition of heterotrophic plant tissue culture, most of them can't synthesize sugar by photosynthesis, so sugar (usually sucrose) needs to be added to the culture medium as a carbon source and energy substance, and sugar also plays an important role in maintaining the osmotic pressure of the culture medium.

(5) pH value

3. Temperature

Temperature is an important condition for successful plant tissue culture. In plant tissue culture, most of them adopt the optimum temperature and keep the constant temperature to accelerate the growth. Usually, the temperature of 25 plus or minus 2 degrees Celsius is suitable for most plants, but it also varies from species to species. Chrysanthemum tissue culture is 18~22 degrees.

lamplight

Different plants often have different requirements for various conditions. Light is also an important condition for tissue culture, which has a great influence on growth and differentiation. Of course, this is also related to the properties of materials, the conditions of culture medium and the temperature rise caused by light. The light intensity of chrysanthemum cultivation is 1000~4000lx, and the daily light intensity is12 hours.

Where can I buy the culture medium for plant tissue culture? Hello, did you do the experiment in small batch or mass production?

Generally, companies related to tissue culture are selling on the Internet, mainly MS culture medium.

Because different plants have different requirements for culture medium, I personally suggest that you can make it yourself if you can, so that you can know how to improve the formula.

If you really need to buy, please leave your qq or other contact information for detailed discussion.

Welcome to ask, please accept it if you are satisfied, thank you.