What are the purification methods?

Purification methods include distillation, centrifugation and filtration.

Distillation is a separation method using the difference of boiling points of substances. Heat the mixture until one of the components evaporates, and then condense it back to the liquid state, so that a relatively pure substance can be obtained. Centrifugal method uses the difference of centrifugal force to separate the mixture, which can separate the components with higher or lower density in the mixture, thus achieving the purpose of purification.

Filtering method filters out impurities in the mixture through filter paper or other filtering equipment to obtain relatively pure substances. By controlling the solubility of the solute in the solvent, the crystallization method makes the solute crystallize into a solid, and then filters out the solvent to obtain a relatively pure substance. Extraction is to use the solubility difference of different substances in different solvents, so that one component in the mixture is dissolved in a specific solvent, and then relatively pure substances are obtained through separation.

Matters needing attention in purification

1, laboratory operation should pay attention to safety, wear laboratory safety equipment, such as lab clothes, gloves, safety glasses, etc.

2. First, we should choose suitable purification methods, such as affinity chromatography, centrifugal separation and column chromatography. , and choose the appropriate method as needed.

3. For the purification of biological samples, it is necessary to pay attention to the source and treatment methods of the samples to avoid unnecessary components in the samples from interfering with the purification.

4. In the process of purification, the conditions such as pH value, temperature and centrifugal speed should be strictly controlled to ensure the purification effect.

5. The purified sample should be effectively tested and identified to ensure its purity and quality.