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Do you want to boil the flow single staining tube cells?
In flow cytometry, a single stained cells means that only one cell is stained for flow cytometry detection. Cells are usually stained with monoclonal antibodies, which can bind to specific cell surface markers, thus realizing quantitative determination and analysis of cells. In the process of staining, cells react specifically with antibodies to form complexes. These complexes can be excited by the laser of flow cytometry and emit fluorescence signals, thus obtaining many biological characteristics data of cells. Compared with other staining methods, flow cytometry has the advantages of large Qualcomm, high sensitivity and multi-parameter analysis. In the experimental operation of flow cytometry, it is not necessary to boil cells, because boiling cells will destroy the cell structure and the specificity of antibodies. On the contrary, common operation steps include cell collection, washing, staining and flow cytometry detection. These steps can be carried out in the active state of cells to maintain the integrity and function of cells.
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