My wife doesn't agree to immigrate.

Yes, the visa officer will naturally think that your wife has an immigration tendency, and business travel visas will not be issued to such people. So your green card prevented your wife from getting this visa to some extent. If you apply again in a short time, it is likely that nothing will change. Spouses are American green cards and citizens, and such problems often occur when applying for such visas. How old is your wife? Does she have a job in China? You can provide something like this to prove that she has a family, a job and assets in China, and may return to China. But to be honest, it doesn't help much, because if you get married, you have to stay with your spouse, and it's normal for your wife to stay in the United States-that's what they are worried about.