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Imprinted

Cold tracks in cattle.

Methylation analysis of cow cold

Department of Immigration and Refugee Affairs. In the cold track of the first exon, cpg island

was found at 1477 to 1683, as determined by the cpg island prediction software ( www.ebi.ac.uk/ < /p>

Heat embossing/cpgplot/) and primer set are designed for

amplification of sodium bisulfite treated DNA all-inclusive

This aspect work. In addition, the (ton/year) polymorphism

was discovered in 1569 thus enabling discrimination

with the paternal, maternal x chromosome

in this region. Liver and villus sampling

Individual women chose this because these tissues

had previously exhibited biallelic and allelic

expression, respectively, in all female analyses.

Sodium bisulfate sequencing of CPP Island, but

proved difficult; it was evident after analyzing

multiple sequences (>100) that this Possibly

secondary structure or high, abundant repeat sequences

have formed from sodium bisulfate conversion and

inhibit sorting from the past, First

30 ¨ base of c50, pair transcript.

After modification

bicycle parameters, the entire 4 full-length sequences

were from liver samples, not from villi,

which suggested reciprocal methyl CpG

p>

Island between father, mother bovine XIST

DMR. In exon 1 of the XIST locus, a CpG island was

detected at +1477 to +1683, as was determined by

CpG island prediction software (www.ebi.ac.uk/

emboss/cpgplot/) and a primer set was designed for

amplification of bisulfite-treated DNA encompassing

< p>this area. Additionally, a (T/A) polymorphism

was detected at +1569 thereby allowing discrimination

between paternal and maternal X chromosomes

within this region. Liver and chorion samples from

an individual female were chosen since these tissues

had previously exhibited biallelic and monoallelic

expression, respectively, in all females analyzed.

Bisulfite sequencing of the CpG island, however,

proved to be difficult; it became apparent after analyzing

multiple sequences (>100), that possibly a

secondary structure or a high AT-rich repeat sequence

had formed from the bisulfite conversion and

inhibited sequencing from proceeding past the first

30–50 base pairs of the transcript. After modifying

cycling parameters, four full-length sequences were

obtained from the liver sample but not from chorion,

p>

which indicated reciprocal methylation of the CpG

island between the paternal and ma

ternal